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Research Projects د/ أشرف السيد يحيى

Previous and Present Research Projects

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Seroprevalence of Hepatitis B, Hepatitis C and human Immunodeficiency Virus in Al-kharj region صالح أحمد العيفان

Saleh A. Eifan, Shafqt Qamar, Atif Hanif, Khalid S. Al-Zuaag and Abdulkarim F. Alhetheel. 2014, Seroprevalence of Hepatitis B, Hepatitis C and human Immunodeficiency Virus in Al-kharj region. Biomedical Research. Accepted Manuscript No.221111167.

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H1N1 Testing: A Comparative Study between the Rapid BDTM Directigen EZ Flu A+B test and PCR صالح أحمد العيفان

Abdulkarim F. Alhetheel1,, Ahmed M. Albarrag, Ali M. Somily and Saleh A. Eifan. 2014, H1N1 Testing: A Comparative Study between the Rapid BDTM Directigen EZ Flu A B test and PCR. Journal of Pure and Applied Microbiology, Vol. (8) No. 3

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Non-Structural Proteins of Arthropod-Borne Bunyaviruses: Roles and Functions صالح أحمد العيفان

Saleh Eifan , Esther Schnettler , Isabelle Dietrich , Alain Kohl  an Anne-Lie Blomström. 2013,  Non-Structural Proteins of Arthropod-Borne Bunyaviruses: Roles and Functions. Viruses, 5, 2447-2468; doi:10.3390/v5102447 

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, Seroprevalence of Hepatitis B and C virus in premarital screening program in and around Albaha region of Saudi Arabia صالح أحمد العيفان

Saleh A. Eifan and Hamed A.K. Mohamed. 2013, Seroprevalence of Hepatitis B and C virus in premarital screening program in and around Albaha region of Saudi Arabia. Journal of pure & applied microbiology. Vol (8) No.2

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Photocatalytic degradation of organic pollutants in waste water using highly ordered TiO2 nanotubes عمار الفاتح السنوسي
Photocatalytic degradation of organic pollutants in waste water using semiconductor nanomaterials has attracted much attention over the past few decades. Among these various photocatalysts, titanium dioxide (TiO2) has been widely used as traditional photocatalyst for the photodegradation of organic pollutants due to its low cost, excellent charge transport property, nontoxicity and photostability. However, conventional powder photocatalysts are not always suitable for large-scale applications, because they exhibit high recombination efficiency of photogenerated electron–hole pairs, and they are difficult to separate after reaction. In this research, highly ordered TiO2 nanotube arrays will be fabricated by anodic oxidation on Ti substrate as fixed photocatalysts on solid support substrate and their photocatlytic degradation efficiency over methyl orange (MO) will be intensively studied and compared with the traditional photocatalyst TiO2 nanoparticles powder ...
Biosensors based on electropolymerized thin films عمار الفاتح السنوسي
For four decades, biosensors have been the subject of increasing research effort and now constitute a major component of mainstream analytical chemistry for rapid diagnostic of diseases, food quality, environmental pollution or even biological attack due to the growing spectrum of bioterrorism. However, the immobilization of the molecular receptor on the transducer is of key importance for the control and the improvement of the performance of such a sensor. On the other hand, considerable attention has been focused on conducting polymers because of their unusual electronic properties and their great potential for biomolecules immobilization. Several immobilization procedures were used to ensure the coupling of immune species onto conducting polymers, they range from the entrapment within the polymer film to the electrostatic binding. In this project, the coelectropolymerization of a polypyridinyl complex of ruthenium(II) containing three pyrrole groups with the biotin derivative pyrrole provided redox copolymers exhibiting specific adsorption sites for biotinylated biomolecules. The resulting copolymer electrodes will be applied to the immobilization of biotinylated antibodies (anti-human IgG, as a model system) through an avidin bridge. The biosensor based on these immobilization procedures will be interrogated by both electrochemical impedance spectroscopy and cyclic voltmetry. ...
: Impedance Biosensor for Avian Influenza Virus Detection أسامة مختار ورغي

Avian influenza virus infections are a major cause of morbidity in birds and rapid identification of the virus has important clinical, economical and epidemiological implications in animals and human health. Most of the diagnostic technique is based on Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and Enzyme Linked Immunosorbent Assay (ELISA) which are time consuming and expensive. By evolving biosensor for sensitive, direct and rapid detection of Avian influenza virus will lead to the shortening of the timeline. In this project, we will use a high sensitive Electrochemical Impedance Spectroscopy (EIS) technique to develop an inexpensive and miniaturize gold microelectrode biosensors for Highly Pathogenic Avian Influenza (HPAI) virus. We will produce and purify a polyclonal IgG-antibody (anti-H7N1and anti-H5N9) for specific antigen H7N1 and H5N9 recognition. The impedance spectroscopy technique is very sensitive technique to the protein-ligand interaction. The stability, reproducibility and the limit detection of the developed biosensor will be studied and compared with traditional technique such as ELISA and PCR. Different others antigen such as Newcastle virus will be tested for non-specific binding.

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Photocatalytic degradation of organic pollutants in waste water using highly ordered TiO2 nanotubes أسامة مختار ورغي
Photocatalytic degradation of organic pollutants in waste water using semiconductor nanomaterials has attracted much attention over the past few decades. Among these various photocatalysts, titanium dioxide (TiO2) has been widely used as traditional photocatalyst for the photodegradation of organic pollutants due to its low cost, excellent charge transport property, nontoxicity and photostability. However, conventional powder photocatalysts are not always suitable for large-scale applications, because they exhibit high recombination efficiency of photogenerated electron–hole pairs, and they are difficult to separate after reaction. In this research, highly ordered TiO2 nanotube arrays will be fabricated by anodic oxidation on Ti substrate as fixed photocatalysts on solid support substrate and their photocatlytic degradation efficiency over methyl orange (MO) will be intensively studied and compared with the traditional photocatalyst TiO2 nanoparticles powder ...
Biosensors based on electropolymerized thin films أسامة مختار ورغي
For four decades, biosensors have been the subject of increasing research effort and now constitute a major component of mainstream analytical chemistry for rapid diagnostic of diseases, food quality, environmental pollution or even biological attack due to the growing spectrum of bioterrorism. However, the immobilization of the molecular receptor on the transducer is of key importance for the control and the improvement of the performance of such a sensor. On the other hand, considerable attention has been focused on conducting polymers because of their unusual electronic properties and their great potential for biomolecules immobilization. Several immobilization procedures were used to ensure the coupling of immune species onto conducting polymers, they range from the entrapment within the polymer film to the electrostatic binding. In this project, the coelectropolymerization of a polypyridinyl complex of ruthenium(II) containing three pyrrole groups with the biotin derivative pyrrole provided redox copolymers exhibiting specific adsorption sites for biotinylated biomolecules. The resulting copolymer electrodes will be applied to the immobilization of biotinylated antibodies (anti-human IgG, as a model system) through an avidin bridge. The biosensor based on these immobilization procedures will be interrogated by both electrochemical impedance spectroscopy and cyclic voltmetry. ...
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